Chikungunya virus is found in MARCO-expressing floor lymphatic
endothelial cells in the draining lymph node at 8 hpi
UMAP projection shows the fraction of counts aligning to the CHIKV
genome for the 8 hpi timepoint.
UMAP projection shows CHIKV+ cells (>0 CHIKV counts) for the 8
hpi timepoint.
CHIKV counts are shown for cell types with >50 cells for the 8
hpi CHIKV-infected sample.
The fraction of cells identified as CHIKV+ is shown for each cell
type for the 8 hpi timepoint.
MARCO expression is shown for fLECs for mock-infected cells and
CHIKV-infected cells classified as either CHIKV low or CHIKV high. P
values were calculated using a two-sided Wilcoxon rank-sum test with
Bonferroni correction. In the boxplot, the central lines, the box
limits, and the whiskers represent medians, the interquartile range
(IQR), and min/max values that are not outliers, respectively. Outliers
are shown as points and include any values that are more than 1.5× IQR
away from the box.
Madcam1 expression is shown for fLECs as described in E.
Figure S1
CHIKV may target MARCO-expressing LECs for infection
CHIKV signal
UMAP projection shows annotated cell types for the 24 hpi
timepoint.
UMAP projection shows CHIKV sgRNA ratio (sgRNA counts / 5’ counts)
for the 24 hpi timepoint.
The fraction of cells identified as CHIKV-high is shown for each
cell type for the 24 hpi timepoint.
CHIKV sgRNA ratio is shown for cells with >0 sgRNA counts and
>0 5’ counts. Cell types with >10 cells are shown.
The correlation between CHIKV sgRNA ratio and QC metrics is shown
for CHIKV-high cells.